Protein oxidative damage and redox imbalance induced by ionising radiation in CHO cells.
Identifieur interne : 000232 ( Main/Exploration ); précédent : 000231; suivant : 000233Protein oxidative damage and redox imbalance induced by ionising radiation in CHO cells.
Auteurs : Donatella Pietraforte [Italie] ; Eleonora Paulicelli [Italie] ; Clarice Patrono [Italie] ; Lucrezia Gambardella [Italie] ; Giuseppe Scorza [Italie] ; Antonella Testa [Italie] ; Paola Fattibene [Italie]Source :
- Free radical research [ 1029-2470 ] ; 2018.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Glutaredoxins, Glutathione Peroxidase, Glutathione Reductase, Reactive Oxygen Species.
- Animals, CHO Cells, Cricetulus, Oxidation-Reduction, Radiation, Ionizing.
Abstract
Reactive oxygen species (ROS) are important mediators of the cytotoxicity induced by the direct reaction of ionising radiation (IR) with all critical cellular components, such as proteins, lipids, and nucleic acids. The derived oxidative damage may propagate in exposed tissues in a dose- and spatiotemporal dependent manner to other cell compartments, affecting intracellular signalling, and cell fate. To understand how cell damage is induced, we studied the oxidative events occurring immediately after cell irradiation by analysing the fate of IR-derived ROS, the intracellular oxidative damage, and the modification of redox environment accumulating in Chinese hamster ovary (CHO) within 1 h after cell irradiation (dose range 0-10 Gy). By using the immuno-spin trapping technique (IST), spectrophotometric methods, and electron paramagnetic resonance (EPR) spectroscopy, we showed that IR-derived ROS (i) induced an IST-detectable, antioxidant-inhibitable one-electron oxidation of specific intracellular proteins; (ii) altered the glutathione (GSH) content (which was found to increase below 2 Gy, and decrease at higher doses, leading to a redox imbalance); (iii) decreased glutathione peroxidase and glutaredoxin activity; (iv) modified neither glutathione reductase nor thioredoxin reductase activity; (v) were detected by spin trapping technique, but adduct intensity decreased due to cell competition for ROS; and (vi) induced no EPR-detectable radicals assignable to oxidised cellular components. In conclusion, our results showed that IR generated an early high oxidising potential (protein radical intermediates, redox imbalance, modified redox enzyme activity) in irradiated cells potentially able to propagate the damage and induce oxidative modification of secondary targets.
DOI: 10.1080/10715762.2018.1446529
PubMed: 29546780
Affiliations:
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Institute of Health , Rome </wicri:regionArea><wicri:noRegion>Rome </wicri:noRegion></affiliation></author><author><name sortKey="Paulicelli, Eleonora" sort="Paulicelli, Eleonora" uniqKey="Paulicelli E" first="Eleonora" last="Paulicelli">Eleonora Paulicelli</name><affiliation wicri:level="1"><nlm:affiliation>a Core Facilities, EPR Area, Italian Institute of Health , Rome , Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>a Core Facilities, EPR Area, Italian Institute of Health , Rome </wicri:regionArea><wicri:noRegion>Rome </wicri:noRegion></affiliation></author><author><name sortKey="Patrono, Clarice" sort="Patrono, Clarice" uniqKey="Patrono C" first="Clarice" last="Patrono">Clarice Patrono</name><affiliation wicri:level="1"><nlm:affiliation>c Division of Health Protection Technologies , Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA) , Rome , Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>c Division of Health Protection Technologies , Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA) , Rome </wicri:regionArea><wicri:noRegion>Rome </wicri:noRegion></affiliation></author><author><name sortKey="Gambardella, Lucrezia" sort="Gambardella, Lucrezia" uniqKey="Gambardella L" first="Lucrezia" last="Gambardella">Lucrezia Gambardella</name><affiliation wicri:level="1"><nlm:affiliation>b Center for Gender-Specific Medicine , Biomarkers Unit, Italian Institute of Health , Rome , Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>b Center for Gender-Specific Medicine , Biomarkers Unit, Italian Institute of Health , Rome </wicri:regionArea><wicri:noRegion>Rome </wicri:noRegion></affiliation></author><author><name sortKey="Scorza, Giuseppe" sort="Scorza, Giuseppe" uniqKey="Scorza G" first="Giuseppe" last="Scorza">Giuseppe Scorza</name><affiliation wicri:level="1"><nlm:affiliation>a Core Facilities, EPR Area, Italian Institute of Health , Rome , Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>a Core Facilities, EPR Area, Italian Institute of Health , Rome </wicri:regionArea><wicri:noRegion>Rome </wicri:noRegion></affiliation></author><author><name sortKey="Testa, Antonella" sort="Testa, Antonella" uniqKey="Testa A" first="Antonella" last="Testa">Antonella Testa</name><affiliation wicri:level="1"><nlm:affiliation>c Division of Health Protection Technologies , Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA) , Rome , Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>c Division of Health Protection Technologies , Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA) , Rome </wicri:regionArea><wicri:noRegion>Rome </wicri:noRegion></affiliation></author><author><name sortKey="Fattibene, Paola" sort="Fattibene, Paola" uniqKey="Fattibene P" first="Paola" last="Fattibene">Paola Fattibene</name><affiliation wicri:level="1"><nlm:affiliation>a Core Facilities, EPR Area, Italian Institute of Health , Rome , Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>a Core Facilities, EPR Area, Italian Institute of Health , Rome </wicri:regionArea><wicri:noRegion>Rome </wicri:noRegion></affiliation></author></analytic><series><title level="j">Free radical research</title><idno type="eISSN">1029-2470</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>CHO Cells (MeSH)</term><term>Cricetulus (MeSH)</term><term>Glutaredoxins (metabolism)</term><term>Glutathione Peroxidase (metabolism)</term><term>Glutathione Reductase (metabolism)</term><term>Oxidation-Reduction (MeSH)</term><term>Radiation, Ionizing (MeSH)</term><term>Reactive Oxygen Species (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Cellules CHO (MeSH)</term><term>Cricetulus (MeSH)</term><term>Espèces réactives de l'oxygène (métabolisme)</term><term>Glutarédoxines (métabolisme)</term><term>Glutathione peroxidase (métabolisme)</term><term>Glutathione reductase (métabolisme)</term><term>Oxydoréduction (MeSH)</term><term>Rayonnement ionisant (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glutaredoxins</term><term>Glutathione Peroxidase</term><term>Glutathione Reductase</term><term>Reactive Oxygen Species</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Espèces réactives de l'oxygène</term><term>Glutarédoxines</term><term>Glutathione peroxidase</term><term>Glutathione reductase</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>CHO Cells</term><term>Cricetulus</term><term>Oxidation-Reduction</term><term>Radiation, Ionizing</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules CHO</term><term>Cricetulus</term><term>Oxydoréduction</term><term>Rayonnement ionisant</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Reactive oxygen species (ROS) are important mediators of the cytotoxicity induced by the direct reaction of ionising radiation (IR) with all critical cellular components, such as proteins, lipids, and nucleic acids. The derived oxidative damage may propagate in exposed tissues in a dose- and spatiotemporal dependent manner to other cell compartments, affecting intracellular signalling, and cell fate. To understand how cell damage is induced, we studied the oxidative events occurring immediately after cell irradiation by analysing the fate of IR-derived ROS, the intracellular oxidative damage, and the modification of redox environment accumulating in Chinese hamster ovary (CHO) within 1 h after cell irradiation (dose range 0-10 Gy). By using the immuno-spin trapping technique (IST), spectrophotometric methods, and electron paramagnetic resonance (EPR) spectroscopy, we showed that IR-derived ROS (i) induced an IST-detectable, antioxidant-inhibitable one-electron oxidation of specific intracellular proteins; (ii) altered the glutathione (GSH) content (which was found to increase below 2 Gy, and decrease at higher doses, leading to a redox imbalance); (iii) decreased glutathione peroxidase and glutaredoxin activity; (iv) modified neither glutathione reductase nor thioredoxin reductase activity; (v) were detected by spin trapping technique, but adduct intensity decreased due to cell competition for ROS; and (vi) induced no EPR-detectable radicals assignable to oxidised cellular components. In conclusion, our results showed that IR generated an early high oxidising potential (protein radical intermediates, redox imbalance, modified redox enzyme activity) in irradiated cells potentially able to propagate the damage and induce oxidative modification of secondary targets.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29546780</PMID><DateCompleted><Year>2018</Year><Month>09</Month><Day>25</Day></DateCompleted><DateRevised><Year>2018</Year><Month>09</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1029-2470</ISSN><JournalIssue CitedMedium="Internet"><Volume>52</Volume><Issue>4</Issue><PubDate><Year>2018</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Free radical research</Title><ISOAbbreviation>Free Radic Res</ISOAbbreviation></Journal><ArticleTitle>Protein oxidative damage and redox imbalance induced by ionising radiation in CHO cells.</ArticleTitle><Pagination><MedlinePgn>465-479</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1080/10715762.2018.1446529</ELocationID><Abstract><AbstractText>Reactive oxygen species (ROS) are important mediators of the cytotoxicity induced by the direct reaction of ionising radiation (IR) with all critical cellular components, such as proteins, lipids, and nucleic acids. The derived oxidative damage may propagate in exposed tissues in a dose- and spatiotemporal dependent manner to other cell compartments, affecting intracellular signalling, and cell fate. To understand how cell damage is induced, we studied the oxidative events occurring immediately after cell irradiation by analysing the fate of IR-derived ROS, the intracellular oxidative damage, and the modification of redox environment accumulating in Chinese hamster ovary (CHO) within 1 h after cell irradiation (dose range 0-10 Gy). By using the immuno-spin trapping technique (IST), spectrophotometric methods, and electron paramagnetic resonance (EPR) spectroscopy, we showed that IR-derived ROS (i) induced an IST-detectable, antioxidant-inhibitable one-electron oxidation of specific intracellular proteins; (ii) altered the glutathione (GSH) content (which was found to increase below 2 Gy, and decrease at higher doses, leading to a redox imbalance); (iii) decreased glutathione peroxidase and glutaredoxin activity; (iv) modified neither glutathione reductase nor thioredoxin reductase activity; (v) were detected by spin trapping technique, but adduct intensity decreased due to cell competition for ROS; and (vi) induced no EPR-detectable radicals assignable to oxidised cellular components. In conclusion, our results showed that IR generated an early high oxidising potential (protein radical intermediates, redox imbalance, modified redox enzyme activity) in irradiated cells potentially able to propagate the damage and induce oxidative modification of secondary targets.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pietraforte</LastName><ForeName>Donatella</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>a Core Facilities, EPR Area, Italian Institute of Health , Rome , Italy.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>b Center for Gender-Specific Medicine , Biomarkers Unit, Italian Institute of Health , Rome , Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Paulicelli</LastName><ForeName>Eleonora</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>a Core Facilities, EPR Area, Italian Institute of Health , Rome , Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Patrono</LastName><ForeName>Clarice</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>c Division of Health Protection Technologies , Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA) , Rome , Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gambardella</LastName><ForeName>Lucrezia</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>b Center for Gender-Specific Medicine , Biomarkers Unit, Italian Institute of Health , Rome , Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Scorza</LastName><ForeName>Giuseppe</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>a Core Facilities, EPR Area, Italian Institute of Health , Rome , Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Testa</LastName><ForeName>Antonella</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>c Division of Health Protection Technologies , Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA) , Rome , Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fattibene</LastName><ForeName>Paola</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>a Core Facilities, EPR Area, Italian Institute of Health , Rome , Italy.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>03</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Free Radic Res</MedlineTA><NlmUniqueID>9423872</NlmUniqueID><ISSNLinking>1029-2470</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D054477">Glutaredoxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.9</RegistryNumber><NameOfSubstance UI="D005979">Glutathione Peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.8.1.7</RegistryNumber><NameOfSubstance UI="D005980">Glutathione Reductase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016466" MajorTopicYN="N">CHO Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003412" MajorTopicYN="N">Cricetulus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054477" MajorTopicYN="N">Glutaredoxins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005979" MajorTopicYN="N">Glutathione Peroxidase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005980" MajorTopicYN="N">Glutathione Reductase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011839" MajorTopicYN="Y">Radiation, Ionizing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Ionising radiation</Keyword><Keyword MajorTopicYN="N">antioxidant systems</Keyword><Keyword MajorTopicYN="N">electron paramagnetic resonance</Keyword><Keyword MajorTopicYN="N">immuno-spin trapping</Keyword><Keyword MajorTopicYN="N">reactive oxidising species</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>3</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>9</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>3</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29546780</ArticleId><ArticleId IdType="doi">10.1080/10715762.2018.1446529</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Italie</li></country></list><tree><country name="Italie"><noRegion><name sortKey="Pietraforte, Donatella" sort="Pietraforte, Donatella" uniqKey="Pietraforte D" first="Donatella" last="Pietraforte">Donatella Pietraforte</name></noRegion><name sortKey="Fattibene, Paola" sort="Fattibene, Paola" uniqKey="Fattibene P" first="Paola" last="Fattibene">Paola Fattibene</name><name sortKey="Gambardella, Lucrezia" sort="Gambardella, Lucrezia" uniqKey="Gambardella L" first="Lucrezia" last="Gambardella">Lucrezia Gambardella</name><name sortKey="Patrono, Clarice" sort="Patrono, Clarice" uniqKey="Patrono C" first="Clarice" last="Patrono">Clarice Patrono</name><name sortKey="Paulicelli, Eleonora" sort="Paulicelli, Eleonora" uniqKey="Paulicelli E" first="Eleonora" last="Paulicelli">Eleonora Paulicelli</name><name sortKey="Pietraforte, Donatella" sort="Pietraforte, Donatella" uniqKey="Pietraforte D" first="Donatella" last="Pietraforte">Donatella Pietraforte</name><name sortKey="Scorza, Giuseppe" sort="Scorza, Giuseppe" uniqKey="Scorza G" first="Giuseppe" last="Scorza">Giuseppe Scorza</name><name sortKey="Testa, Antonella" 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